[PDF] Meta-topolin: an alternative for the prevention of oxidative stress in sugarcane micropropagation | Semantic Scholar (2024)

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It is possible to regenerate shoots in M. urundeuva from the cotyledonary node using BAP in association with NAA, and all treatments containing this cytokinin showed callus formation.

ZEA and 2iP increased chlorophyll, carotenoids and total soluble sugar contents in micropropagated shoots and strengthened plant defense against oxidative stress in tissue culture.

This study describes the maintenance of germplasm in vitro and establishment of new viable population in its original habitats by restoring existing sites of this critically endangered jewel orchid.

Plants regenerated from mTTHP or mTR treatments demonstrated a high capacity for energy dissipation in comparison to TDZ-regenerated plants with low photochemical quenching, PSII quantum efficiency and non-photochemicalQuenching.

It was found that Pelargonium species varying in their susceptibility to senescence differ in H 2 O 2 production and antioxidant enzymes activities, and meta -topolin was also the most effective in shoot formation.

Quantification of 50 endogenous cytokinins, using a recently developed UPLC-ESI(+)-MS/MS method, showed significant differences in the cytokinin metabolites (especially different glucosides) in explants cultivated on media supplemented with BA or mT.

The consistent increases in activities of superoxide dismutase, catalase, glutathione reductase, and ascorbate peroxidase during acclimatization indicate that mT raised plantlets response well to the stress induced by ex vitro conditions.

The results support the possible use of topolins as an alternative to BA for Cavendish banana tissue culture and requires a more stringent investigation as the results obtained in this investigation could have been influenced by carry-over effects from the initial cultures.

It may be concluded that only 0.1mgL−1 BA concentration combined with NAA could play a direct role in reducing the level of free radicals and phenolic production associated with proliferation capacity of grape cells under in vitro conditions.

The morphogenic response of intercalary meristems present in shoots from sprouting saffron corms when cultured in media with different cytokinins is evaluated to suggest a possible use of antioxidant enzyme activities as a parameter that could be use to evaluate the efficiency of micropropagation protocols for saffer and even in other species.

A good correlation was found between antioxidant capacity and total soluble phenolics present in Cistus extracts, pointing to a prominent role played by this flavonoid in C. heterophyllus defence against oxidative stress, which affects the in vitro performance of this species.

Nine of the enzyme activities were eliminated with cyanide treatment suggesting that they may be cupro-zinc enzymes, whereas one was cyanide-resistant and may be a manganese enzyme.

In vitro culture protocols for naranjilla apical buds, hypocotyls and petioles are reported, improving on previously reported protocols for this species by using alternative explant types and regeneration protocols.

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